In vitro antimicrobial activity of the triantibiotic paste, according to the time of application and storage
Keywords:
triantibiotic paste, antimicrobial activity, Enterococcus faecalis.Abstract
Introduction: Intracanal medication with antibiotics is used to ensure the success of treatments. However, no studies evaluating the efficacy of triantibiotic paste after several hours of preparation have been reported.
Objective: To evaluate the antimicrobial activity of the triantibiotic paste against Enterococcus faecalis, according to the time of application and storage of the components used for its preparation.
Methods: An experimental in vitro study was carried out in the microbiology laboratory of Universidad Nacional Mayor de San Marcos. The sample consisted of three colonies of Enterococcus faecalis, formed in bile-esculin agar. On three specific days (0, 14 and 28), the antimicrobial activity of the conventional (ciprofloxacin/metronidazole/minocycline) and modified (cefaclor/metronidazole/minocycline) paste was evaluated, measuring (mm) the inhibition zones. The freshly obtained components were used to prepare the paste on day 0, and the stored components (powdered antibiotics kept in amber glass bottles at room temperature) were used on days 14 and 28. Two interventions were performed on each specific day (morning and afternoon). Freshly prepared pastes were used in the morning (immediate application), while pastes stored for 6 hours (delayed application) were used in the afternoon.
Results: On day 0, it was found that the modified triantibiotic paste of immediate application had higher antimicrobial activity than the one of delayed application (p = 0.046). On day 28, the conventional triantibiotic paste for immediate application showed higher antimicrobial activity than that for delayed application (p = 0.049). Pasta prepared with fresh components (day 0) had higher antimicrobial activity than pasta prepared with components stored for 14 and 28 days.
Conclusions: The application time of the triantibiotic paste and the storage times of the components could influence the antimicrobial activity for the eradication of Enterococcus faecalis.
Downloads
References
Alghamdi F, Shakir M. The Influence of Enterococcus faecalis as a Dental Root Canal Pathogen on Endodontic Treatment: A Systematic Review. Cureus. 2020;12(3):e7257-e. DOI: 10.7759/cureus.7257
Prada I, Micó Muñoz P, Giner Lluesma T, Collado Castellano N, Manzano Saiz A. Influence of microbiology on endodontic failure. Literature review. Med Oral Patol Oral Cir Bucal. 2019 May 1;24(3):e364-e372. DOI: 10.4317/medoral.22907
Dioguardi M, Di Gioia G, Illuzzi G, Arena C, Caponio VCA, Caloro GA, Zhurakivska K, Adipietro I, Troiano G, Lo Muzio L. Inspection of the Microbiota in Endodontic Lesions. Dent J (Basel). 2019 May 1;7(2):47. DOI: 10.3390/dj7020047
Makandar S, Noorani T. Triple antibiotic paste - Challenging intracanal medicament: A systematic review. J Int Oral Health. 2020;12(3):189-96. DOI: 10.4103/JIOH.JIOH_213_19
Saatchi M, Shokraneh A, Navaei H, Maracy MR, Shojaei H. Antibacterial effect of calcium hydroxide combined with chlorhexidine on Enterococcus faecalis: a systematic review and meta-analysis. J Appl Oral Sci. 2014;22(5):356-65. DOI: 10.1590/1678-775720140032
Wang Y, Guo L Y, Fang H Z, Zou W L, Yang Y M, Gao Y, et al. An in vitro study on the efficacy of removing calcium hydroxide from curved root canal systems in root canal therapy. Int J Oral Sci. 2017;9(2):110-6. DOI: 10.1038/ijos.2017.14
Jia L, Zhang X, Shi H, Li T, Lv B, Xie M. The Clinical Effectiveness of Calcium Hydroxide in Root Canal Disinfection of Primary Teeth: A Meta-Analysis. Med Sci Monit. 2019;25:2908-16. DOI: 10.12659/MSM.913256
Alireza adl, Sabie Hamedi, Mahdi Sedigh Shams, et al. The Ability of Triple Antibiotic Paste and Calcium Hydroxide in Disinfection of Dentinal Tubules. Iran Endod J. 2014; 9(2): 123-26. DOI: 10.22037/iej.v9i2.4816
Kishen A, George S, Kumar R. Enterococcus faecalis-mediated biomineralized biofilm formation on root canal dentine in vitro. J Biomed Mater Res A. 2006 May;77(2):406-15. DOI: 10.1002/jbm.a.30622
Hoshino E, Kurihara Ando N, Sato I, Uematsu H, Sato M, Kota K, et al. In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J. 1996;29(2):125-30. DOI: 10.1111/j.1365-2591.1996.tb01173.x
Alam T, Nakazawa F, Nakajo K, Uematsu H, Hoshino E. Susceptibility of Enterococcus faecalis to a Combination o Antibacterial Drugs (3Mix) in vitro. J Oral Biosci. 2005;47(4):315-20. DOI: 10.1016/S1349-0079(05)80014-3
Mohammadi Z, Jafarzadeh H, Shalavi S, Yaripour S, Sharifi F, Kinoshita J-I. A Review on Triple Antibiotic Paste as a Suitable Material Used in Regenerative Endodontics. Iran Endod J. 2018;13(1):1-6. DOI: 10.22037/iej.v13i1.17941.
Dhillon JS, Amita, Saini SK, Bedi HS, Ratol SS, & Gill B. Healing of a large periapical lesion using triple antibiotic paste and intracanal aspiration in nonsurgical endodontic retreatment. Indian J Dent. 2004; 5(3):161-5. DOI: 10.4103/0975-962X.140843
Asnaashari M, Eghbal MJ, Sahba Yaghmayi A, Shokri M, Azari Marhabi S. Comparison of antibacterial effects of photodynamic therapy, modified triple antibiotic paste and calcium hydroxide on root canals infected with enterococcus faecalis: an in vitro study. J Lasers Med Sci. 2019;10(Suppl 1):S23-9. DOI: 10.15171/jlms.2019.S5
Dewi A, Upara C, Krongbaramee T, Louwakul P, Srisuwan T, Khemaleelakul S. Optimal antimicrobial concentration of mixed antibiotic pastes in eliminating Enterococcus faecalis from root dentin. Aust Endod J. 2020. DOI: 10.1111/aej.12437
Pharmaceutical Preparations. In European Pharmacopoeia 9.5. Supplement; Council of Europe: Strasbourg, France, 2018; pp. 5569-71.
Falconer JR, Steadman KJ. Extemporaneously compounded medicines. Aust Prescr. 2017;40(1):5-8. DOI: 10.18773/austprescr.2017.001
Nagel K, Ali F, Al Khudari S, Khan A, Patel K, Patel N, Desai A. Extemporaneous compounding of medicated ointments. Int J Pharm Compd. 2010;14(6):472-8. PMID: 23965650
Dasari V, Maroli S, Chowdary L, Karukola R, Premakumar SH, Vusurumarthi V. An in vivo study evaluating lesion sterilization and tissue repair 3 MIX-MP noninstrumentation endodontic treatment as an alternative to conventional endodontic retreatment. CHRISMED J Health Res 2016;3:284-7. DOI: 10.4103/2348-3334.190579 20.
Sain S, Reshmi J, Anandaraj S, George S, S Issac J, A John S. Lesion Sterilization and Tissue Repair-Current Concepts and Practices. Int J Clin Pediatr Dent. 2018;11(5):446-50. DOI: 10.5005/jp-journals-10005-1555
Bravo. S. Efectos antibacterianos de las combinaciones alternativas de la droga 3Mix y MP sobre bacterias prevalentes en necrosis pulpar. [Tesis Pregrado]. Lima, Universidad Nacional Mayor de San Marcos; 2015. https://cybertesis.unmsm.edu.pe/handle/20.500.12672/4459
Aucamp M, Milne M. The physical stability of drugs linked to quality-by-design (QbD) and in-process technology (PAT) perspectives. Eur J Pharm Sci. 2019; 139:105057. DOI: 10.1016/j.ejps.2019.105057
Rendón Laura M., Sara Lopera, Naira Y. Valencia. Estabilidad de los medicamentos después del primer uso. J Pharm Pharmacogn Res. 2018[acceso 15/08/2021];6(5):412-23. Disponible en: https://jppres.com/jppres/estabilidad-de-los-medicamentos-despues-del-primer-uso/
Makeen HA, Pancholi SS, Alhazmi HA, Ezzi AA, Hazzazi AJA, Meraya AM. Stability testing of extemporaneous preparation of methyl salicylate ointment. J Health Spec 2018;6:72-6. DOI: 10.4103/jhs.JHS_14_18
Published
How to Cite
Issue
Section
License
Authors retain all rights to their works, which they can reproduce and distribute as long as they cite the primary source of publication.
The Rev Cubana Estomatol is subject to the Creative Commons Attribution-Non-Commercial 4.0 International License (CC BY-NC 4.0) and follows the publication model of SciELO Publishing Schema (SciELO PS) for publication in XML format.
You are free to:
- Share — copy and redistribute the material in any medium or format.
- Adapt — remix, transform, and build upon the material.
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
- You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
- No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
Download PDF
