Antibacterial activity of Camellia sinensis versus propolis against Streptococcus mutans

Authors

  • Cesar Felix Cayo Rojas Universidad Inca Garcilaso de la Vega, Instituto de Investigación. Lima. Universidad Inca Garcilaso de la Vega, Facultad de Estomatología. Lima. Universidad Nacional Federico Villarreal, Escuela Universitaria de Posgrado. Lima. https://orcid.org/0000-0002-5560-7841
  • Luis Adolfo Cervantes Ganoza Universidad Inca Garcilaso de la Vega, Facultad de Estomatología. Lima. https://orcid.org/0000-0001-6090-6750

Keywords:

Camellia sinensis (green tea), propolis, chlorhexidine.

Abstract

Introduction: Green tea (Camellia sinensis) and propolis contain flavonoids which inhibit the growth, metabolism and co-aggregation of Streptococcus mutans, the main causative agent of dental caries.

Objective: Evaluate the antibacterial activity in vitro of 10% and 20% green tea (Camellia sinensis) ethanolic extract versus 10% and 20% propolis ethanolic extract against the growth of Streptococcus mutans (ATCC 25175) strains.

Methods: An in vitro experimental prospective longitudinal comparative study was conducted. The study universe was 90 diffusion disks and the sample was 15 disks soaked up in green tea (Camellia sinensis) or propolis at various concentrations, 0.12% aqueous chlorhexidine and distilled water. Sample size was estimated by the comparison of means formula after conducting a pilot study. The diffusion disks soaked up in the substances were placed on Mueller Hinton agar planted with Streptococcus mutans (ATCC 25175) and the width of the inhibition haloes was measured at 24 h and 48 h. Nonparametric Kruskal-Wallis comparison tests and the Wilcoxon rank test were performed.

Results: The maximum width of the inhibition halo achieved by 0.12% aqueous chlorhexidine, 20% green tea (Camellia sinensis) ethanolic extract, and 20% propolis ethanolic extract at 24 h was 10.64 mm ± 0.924 mm, 6.82 mm ± 0.982 mm and 8.36 mm ± 1.286 mm, respectively. The 20% green tea (Camellia sinensis) ethanolic extract showed statistically significant differences with respect to the 20% propolis ethanolic extract, both at 24 h (p= 0.013) and at 48 h (p= 0.011).

Conclusions: The 20% propolis ethanolic extract displays greater antibacterial activity against the growth of Streptococcus mutans (ATCC 25175) strains than the 10% and 20% green tea (Camellia sinensis) ethanolic extract. This activity decreases with the passing of time.

Downloads

Download data is not yet available.

Author Biographies

Cesar Felix Cayo Rojas, Universidad Inca Garcilaso de la Vega, Instituto de Investigación. Lima. Universidad Inca Garcilaso de la Vega, Facultad de Estomatología. Lima. Universidad Nacional Federico Villarreal, Escuela Universitaria de Posgrado. Lima.

Doctor en Odontología, cirujano dentista, magíster en docencia universitaria y gestión educativa, especialista en carielogía y endodoncia; egresado del doctorado en Odontología UNFV, docente de ciencias básicas a nivel de pregrado y posgrado.
Docente en la UNFV - UAP - UIGV - UNJFSC - CEPREVI, asesor de tesis, autor de varias artículos científicos y un libro de Histología y Embriología

Luis Adolfo Cervantes Ganoza, Universidad Inca Garcilaso de la Vega, Facultad de Estomatología. Lima.

Doctor en Salud Pública, magíster en Estomatología, cirujano dentista

References

Organización Mundial de la Salud. Estrategia de la OMS sobre medicina tradicional 2014-2023. 2013 [acceso 14/07/2019]. Disponible en: https://apps.who.int/iris/handle/10665/95008

Hegde RJ, Kamath S. Comparison of the Streptococcus mutans and Lactobacillus colony count changes in saliva following chlorhexidine (0.12%) mouth rinse, combination mouth rinse, and green tea extract (0.5%) mouth rinse in children. J Indian Soc Pedod Prev Dent [Internet]. 2017 [acceso 14/01/2019];35(2):150-5. Disponible en: https://doi.org/10.4103/JISPPD.JISPPD_13_17

Mohan PU, Uloopi KS, Vinay C, Rao RC. In vivo comparison of cavity disinfection efficacy with APF gel, Propolis, Diode Laser and 2% chlorhexidine in primary teeth. Contemp Clin Dent [Internet] 2016 [acceso 14/01/2019];7(1):45-50. Disponible en: https://doi.org/10.4103/0976-237X.177110

Anita P, Sivasamy S, Madan Kumar PD, Balan IN, Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharma [Internet]. 2015 [acceso 14/01/2019];6(1):35-9. Disponible en: https://www.jbclinpharm.org/articles/in-vitro-antibacterial-activity-of-camellia-sinensis-extract-against-cariogenic-microorganisms.html

Libério SA, Pereira ALA, Araújo MJAM, Dutra RP, Nascimento FRF, Monteiro-Neto V, et al. The potential use of propolis as a cariostatic agent and its actions on mutans group streptococci. Journal of Ethnopharmacology [Internet]. 2009 [acceso 14/01/2019];125(1):1-9. Disponible en: https://doi.org/10.1016/j.jep.2009.04.047

Moreno Z, Martínez P, Figueroa J. Efecto antimicrobiano in vitro de propóleos argentinos, colombianos y cubano sobre Streptococcus mutans ATCC 25175. NOVA 2007 [acceso 14/01/2019];5(7):70-5. Disponible en: http://www.redalyc.org/pdf/411/41150710.pdf

Mayta F, Sacsaquispe S. Evaluación in vitro del efecto antibacteriano del extracto etanólico de propóleo de Oxapampa-Perú sobre cultivos de Streptococcus mutans (ATCC 25175) y Staphylococcus aureus (ATCC 25923). Rev Estomatol Herediana [Internet]. 2010 [acceso 12/07/2019];20(1):19-24. Disponible en: https://doi.org/10.20453/reh.v20i1.1777

Sarkar P, Kumar S, Das S, Airen B. Anticariogenic potential of Camellia sinensis on Streptococcus mutans - An in vitro study. J Evolution Med Dent Sci [Internet]. 2019 [acceso 20/09/2019];8(16):1268-72. DOI: 10.14260/jemds/2019/283. Disponible en: https://www.jemds.com/data_pdf/pubali%20sarkar-apr-22-.pdf

Arévalo A, Gómez P. Efectividad antibacteriana de productos naturales frente a microorganismos patógenos de la flora oral. In Crescendo Ciencias de la Salud. 2016;2(2):530-7.

Akca AE, Akca G, Toksoy Topçu F, Macit E, Pikdöken L, Şerif I. The Comparative Evaluation of the Antimicrobial Effect of Propolis with Chlorhexidine against Oral Pathogens: An In Vitro Study. Biomed Res Int [Internet]. 2016 [acceso 20/09/2019]. Disponible en: https://doi.org/10.1155/2016/3627463

Lamont RJ, Hajisshengallis GN, Jenkinson HF. Microbiología e Inmunología Oral. México: El Manual Moderno; 2015.

Padilla K. Efecto antibacteriano de una infusión de Camellia sinensis (té verde) usada como colutorio sobre placa bacteriana y saliva. Pueblo Continente. 2015;24(2):349-356.

Fan D, Fan K, Yu C, Lu Y, Wang X. Tea polyphenols dominate the short-term tea (Camellia sinensis) leaf litter decomposition. J Zhejiang Univ Sci B [Internet]. 2017 [acceso 20/09/2019];18:(2):99-108. Disponible en: https://doi.org/10.1631/jzus.B1600044

Baladia E, Basulto J, Manera M, Martínez R, Calbet D. Efecto del consumo de té verde o extractos de té verde en el peso y en la composición corporal: revisión sistemática y metaanálisis. Nutr Hosp [Internet]. 2014 [acceso 20/09/2019];29(3):479-90. Disponible en: http://dx.doi.org/10.3305/NH.2014.29.3.7118

Khurshid Z, Zafar MS, Zohaib S, Najeeb S, Naseem M. Green Tea (Camellia Sinensis): Chemistry and Oral Health. Open Dent J [Internet]. 2016 [acceso 20/09/2019];10(Suppl-1):166-73. doi: 10.2174 / 1874210601610010166. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911733/

Veloz JJ, Saavedra N, Lillo A, Alvear M, Barrientos L, Salazar L. Antibiofilm Activity of Chilean Propolis on Streptococcus mutans Is Influenced by the Year of Collection. Biomed Res Int [Internet]. 2015 [acceso 20/09/2019]. Disponible en: https://doi.org/10.1155/2015/291351

Kawarai T, Narisawa N, Yoneda S, Tsutsumi Y, Ishikawa J, Hoshino Y, et al. Inhibition of biofilm formation of Streptococcus mutans using Assam tea extracts compared to green tea. Arch Oral Biol [Internet]. 2016 [cited in 2019 Set 20];68:73-82. Disponible en: https://doi.org/10.1016/j.archoralbio.2016.04.002

Ren Z, Chen L, Li J, Li Y. Inhibition of Streptococcus mutans polysaccharide synthesis by molecules targeting glycosyltransferase activity. J Microbiol Oral [Internet]. 2016 [cited in 2019 Set 20];8(1). DOI: 10.3402 / jom.v8.31095

Downloads

Published

2020-03-21

How to Cite

1.
Cayo Rojas CF, Cervantes Ganoza LA. Antibacterial activity of Camellia sinensis versus propolis against Streptococcus mutans. Rev Cubana Estomatol [Internet]. 2020 Mar. 21 [cited 2025 Feb. 7];57(1):e2967. Available from: https://revestomatologia.sld.cu/index.php/est/article/view/2967

Issue

Vol. 57 No. 1 (2020): JAN- MAR

Section

Research Article

License

Authors retain all rights to their works, which they can reproduce and distribute as long as they cite the primary source of publication.

The Rev Cubana Estomatol is subject to the Creative Commons Attribution-Non-Commercial 4.0 International License (CC BY-NC 4.0) and follows the publication model of SciELO Publishing Schema (SciELO PS) for publication in XML format.

You are free to:

  • Share — copy and redistribute the material in any medium or format.
  • Adapt — remix, transform, and build upon the material.

The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Notices:

  • You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
  • No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.