Decrease in Streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults

Authors

  • Patricio Vildósola Grez Universidad de Chile. Facultad de Odontología, Departamento de Odontología Restauradora. Santiago de Chile, Chile. Universidad Andres Bello. Facultad de Odontología. Santiago de Chile, Chile. https://orcid.org/0000-0002-0649-4334
  • Eduardo Fernandez Universidad de Chile. Facultad de Odontología, Departamento de Odontología Restauradora. Santiago de Chile, Chile. Universidad Autónoma de Chile. Instituto de Ciencias Biomédicas. Santiago de Chile, Chile. https://orcid.org/0000-0002-2616-1510
  • Jose Roberto Cury Saad Universidad Estadual Paulista. Facultad de Odontología, Departamento de Odontología Restauradora. Araraquara. São Paulo, Brazil. Universidad Politécnica y Artística del Paraguay, Paraguay. https://orcid.org/0000-0001-8991-4404
  • Patricia Palma Fluxá Universidad de Chile. Facultad de Microbiología Dental, Departamento de MEdicina y Patología Oral. Santiago de Chile, Chile. https://orcid.org/0000-0002-1841-6798
  • Alain Manuel Chaple Gil Universidad de Ciencias Médicas de La Habana, Facultad de Ciencias Médicas “Victoria de Girón”, Departamento de Estomatología General Integral. La Habana, Cuba https://orcid.org/0000-0002-8571-4429
  • Natalia Acuña Zepeda Universidad de Chile. Facultad de Odontología, Departamento de Odontología Restauradora. Santiago de Chile, Chile. https://orcid.org/0000-0003-0331-9343
  • Gustavo Moncada Cortés Universidad de Chile. Facultad de Odontología, Departamento de Odontología Restauradora. Santiago de Chile, Chile. https://orcid.org/0000-0003-0165-4121

Keywords:

pit and fissure sealants, Streptococcus mutans, permanent dental restoration, composite resins.

Abstract

Introduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk.

Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant.

Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence.

Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05).

Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans.

Downloads

Downloads

Published

2019-11-18

How to Cite

1.
Vildósola Grez P, Fernandez E, Cury Saad JR, Palma Fluxá P, Chaple Gil AM, Acuña Zepeda N, et al. Decrease in Streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults. Rev Cubana Estomatol [Internet]. 2019 Nov. 18 [cited 2025 Jun. 6];56(4):1-12. Available from: https://revestomatologia.sld.cu/index.php/est/article/view/2121

Issue

Vol. 56 No. 4 (2019): OCT- DEC

Section

Research Article

License

Authors retain all rights to their works, which they can reproduce and distribute as long as they cite the primary source of publication.

The Rev Cubana Estomatol is subject to the Creative Commons Attribution-Non-Commercial 4.0 International License (CC BY-NC 4.0) and follows the publication model of SciELO Publishing Schema (SciELO PS) for publication in XML format.

You are free to:

  • Share — copy and redistribute the material in any medium or format.
  • Adapt — remix, transform, and build upon the material.

The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Notices:

  • You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
  • No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.