Irradiación Láser de baja intensidad en cepas de Candida: In vitro
Keywords:
candida, candidiasis oral, laser therapy, Low-Level.Abstract
Objective: To evaluate the effect of specific parameters of low-level laser irradiation on strains of Candida albicans (ATCC 18804), Candida krusei (ATCC 34135) and Candida tropicalis (ATCC 13803).
Methods: The inocula of the three Candida species (1.5 x 106 microorganisms/ml) were irradiated with a gallium-arsenide (GaAs) infrared laser device (Twinflex Evolution, MMO Electronic Equipment, 660 nm, 0.5 nW) at doses (J/cm2): 1.2 (10 sec), 3.7 (30 sec), 7.5 (1min) and 15 (2 min). Following irradiation, the inocula were grown on Petri dishes containing Sabouraud Dextrose culture medium and then incubated in bacteriological incubator at 37 °C. After 48 hours, it was quantified the number of colony-forming units (CFU) and data were statistically analyzed using Friedman’s and Wilcoxon’s tests (α=0.05). All tests were performed in duplicate.
Results: The median values (Q25 - Q75) gathered in the quantification of the strains after laser irradiation at doses (J/cm2) 1.2, 3.7, 7.5 and 15 were, respectively: 35.23 (9.15 - 47.64); 6.79 (1.45 - 6.87); 5.32 (1.39 - 8.15); 6.10 (1.18 - 11.86) and 5.13 (0.99 - 6.25). These results were found to show statistically significant differences according to the dose administered (p<0.05). Nevertheless, it was not possible to identify in the post-hoc tests which group(s) showed significant difference.
Conclusion: Low-intensity laser therapy showed inhibitory effect on Candida strains, and such activity was altered according to the irradiated dose.
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